Sf9 ( IPLB-21)

Origin: fall armyworm (Insecta). Spodoptera frugiperda. Pupal ovary cells.

Invertebrate tissue cultures. Research Application. 1976. p.328

Morphology: small spherical cells with a few fibroblast-like cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46 or IPL-40

serum - FBS 5%

subculture procedure - cell detachment mechanically,

split ratio 1:10, optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: polyploid > 100 chromosomes

Other properties:

virus susceptibility: baculoviruses

Applications: biotechnology, virology

Collections: MWIGG