Origin:Drosophila melanogaster (Insecta), last embryonal cells.
J.Embryol. Exp. Morphol. 1972, 27: 353-365.
Morphology: round, fibroblast-like, epithelial like
Mode of cultivation: monolayer
Conditions for cultivation: medium - C-46
serum - FBS 10%
subculture procedure - cell detachment mechanically, split ratio 1:10,
optimal population density 1.0x106 cells/ml
cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: diploid 2n = 8
Plating efficiency: 20%
virus susceptibility: picornaviruses
Applications: cell biology