S3

Origin: Drosophila melanogaster (Insecta), last embryonal cells.

J.Embryol. Exp. Morphol. 1972, 27: 353-365.

Morphology: round, fibroblast-like, epithelial like

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:10,

optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: diploid 2n = 8

Plating efficiency: 20%

Other properties:

virus susceptibility: picornaviruses

Applications: cell biology

Collections: MWIGG