Origin:Drosophila melanogaster (Insecta). Cells of last embryos and first stage larvae.
J. Embryol. Exp. Morphol. 1972, 27: 353-365
Morphology: round cells
Mode of cultivation: monolayer
Conditions for cultivation: medium - C-46
serum - FBS 10%
subculture procedure - cell detachment mechanically, split ratio 1:10,
optimal population density 1.0x106 cells/ml
cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (G6PD) analysis
Karyology: 60%- diploid, 40%- heteroploid
Plating efficiency: 10 %
virus susceptibility: picornaviruses, VS
Applications: cell biology, somatic cells genetics, virology.