S1

Origin: Drosophila melanogaster (Insecta). Cells of last embryos and first stage larvae.

J. Embryol. Exp. Morphol. 1972, 27: 353-365

Morphology: round cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:10,

optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (G6PD) analysis

Karyology: 60%- diploid, 40%- heteroploid

Plating efficiency: 10 %

Other properties:

virus susceptibility: picornaviruses, VS

Applications: cell biology, somatic cells genetics, virology.

Collections: MWIGG