Mos 20 A
Origin:Mosquito (Insecta), Aedes aegypti. Minced trypsinized larvae of mosquito.
J.Med. Entomol. 1969, 6: 432-439.
Mode of cultivation: monolayer
Conditions for cultivation: medium - C-46
serum - FBS 10%
subculture procedure -cells detachment mechanically, split ratio 1:20,
optimal population density 0.5x106 cells/ml.
cryoconservation - growth medium, 10% DMSO, 1.0-2.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 6
Plating efficiency: 10%
virus susceptibility: yellow fever virus, CPV, SF.
Applications: cell biology, biotechnology, virology.