Mos 20 A

Origin: Mosquito (Insecta), Aedes aegypti. Minced trypsinized larvae of mosquito.

J.Med. Entomol. 1969, 6: 432-439.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure -cells detachment mechanically, split ratio 1:20,

optimal population density 0.5x106 cells/ml.

cryoconservation - growth medium, 10% DMSO, 1.0-2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 6

Plating efficiency: 10%

Other properties:

virus susceptibility: yellow fever virus, CPV, SF.

Applications: cell biology, biotechnology, virology.

Collections: MWIGG