-O4

Origin: Agrothis segetum (Insecta), pupal ovary cells

Deposited in MWIGG, patent of RF

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:10,

optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (SOD) analysis

Karyology: polyploid. > 130 chromosomes

Other properties:

virus susceptibility: baculoviruses

isoenzymes G6PD, LDH

Applications: virology, biotechnology

Collections: MWIGG