Origin:Drosophila melanogaster (Insecta), 6 h embryo.
In vitro. 1970, 6:162.
Morphology: round cells
Mode of cultivation: monolayer
Conditions for cultivation: medium - C-46
serum - FBS 10%
subculture procedure - cell detachment mechanically, split ratio 1:10,
optimal population density 1.0x106 cells/ml
cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule.
Viability after cryoconservation: 90% (0 passage, dye trypan blue).
Sterility: tests for bacteria, fungi and mycoplasma were negative.
Species: karyological analysis
Karyology: diploid 2n = 7 ( XO- cells)
Plating efficiency: 20%
virus susceptibility: picornaviruses
isoenzymes isozymes GPGD
genetical markers: GPRT -
Applications: cell biology, virology, endocrinology