Kc

Origin: Drosophila melanogaster (Insecta), 6 h embryo.

In vitro. 1970, 6:162.

Morphology: round cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:10,

optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule.

Viability after cryoconservation: 90% (0 passage, dye trypan blue).

Sterility: tests for bacteria, fungi and mycoplasma were negative.

Species: karyological analysis

Karyology: diploid 2n = 7 ( XO- cells)

Plating efficiency: 20%

Other properties:

virus susceptibility: picornaviruses

isoenzymes isozymes GPGD

genetical markers: GPRT -

Applications: cell biology, virology, endocrinology

Collections: MWIGG