G2

Origin: Drosophila melanogaster (Insecta). Embryonic cells.

Genetics and Biology of Drosophila. London. 1978. 2: 266

Morphology: round cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure - cell detachment mechanically,

split ratio 1:10, optimal population density 0.5x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0-5.0x106 cells/ml in ampule

Viability after cryoconservation: 80% ( 0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: hypotetraploid

Plating efficiency: 20%

Applications: cell biology

Collections: MWIGG