Origin:Drosophila hydei (Insecta), embryonal cells.
In vitro 1980, 16: 913
Morphology: spindle-shaped cells
Mode of cultivation: monolayer
Conditions for cultivation: medium - C-46
serum - FBS 10%
subculture procedure - cell detachment mechanically, split ratio 1:10,
optimal population density 1.0x106 cells/ml.
cryoconservation - growth medium, 10% DMSO, 2.0x106 cell/ ml in ampule.
Viability after cryoconservation: 80% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: diploid 2n= 12 (XY- chromosomes)
Plating efficiency: 10%
virus susceptibility: picornaviruses
Applications: cell biology, virology.