Dh33

Origin: Drosophila hydei (Insecta), embryonal cells.

In vitro 1980, 16: 913

Morphology: spindle-shaped cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:10,

optimal population density 1.0x106 cells/ml.

cryoconservation - growth medium, 10% DMSO, 2.0x106 cell/ ml in ampule.

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: diploid 2n= 12 (XY- chromosomes)

Plating efficiency: 10%

Other properties:

virus susceptibility: picornaviruses

Applications: cell biology, virology.

Collections: MWIGG