Dh14

Origin: Drosophila Hydei (Insecta), embryonic blastema

In Vitro, 1980, 16: 913

Morphology: round cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:10,

optimal population density 0.5x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 11 (XO -cells)

Plating efficiency: 10%

Applications: cell biology

Collections: MWIGG