Origin:Silkworm, Bombyx mori, ovary cells , clonal line.
Appl. Environ. Microbiol., 1982, 44:227
Morphology: spindle-shapped cells
Mode of cultivation: monolayer
Conditions for cultivation: medium - C-46 or TMN-FH
serum - FBS 10%
subculture procedure - cell detachment mechanically, split ratio 1:5,
optimal population density 1.0x106 cells/ml
cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: Isoenzymological analysis (6 PGD)
Karyology: polyploid. 260- 300 chromosomes.
Plating efficiency: 10%
virus susceptibility: baculoviruses
isoenzymes 6 PGD, LDH, SOD