BmN

Origin: Silkworm, Bombyx mori, ovary cells , clonal line.

Appl. Environ. Microbiol., 1982, 44:227

Morphology: spindle-shapped cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46 or TMN-FH

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:5,

optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: Isoenzymological analysis (6 PGD)

Karyology: polyploid. 260- 300 chromosomes.

Plating efficiency: 10%

Other properties:

virus susceptibility: baculoviruses

isoenzymes 6 PGD, LDH, SOD

Applications: biotechnology

Collections: MWIGG