Bm

Origin: Silkworm. Bombyx mori; larval ovary cells.

Nature, 1967, 216: 613

Morphology: spindle-like cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46 or Grase

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:5,

optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 70% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (PGD) analysis

Karyology: polyploid >100 chromosomes

Plating efficiency: 1%

Other properties:

virus susceptibility: baculoviruses

isoenzymes LDH, ICDG, SOD

Applications: virology, biotechnology.

Collections: MWIGG