85 h 16 Dm Or R (OrR)

Origin: Drosophila melanogaster (Insecta), 12 h embryotic cells.

Proc III International cell culture congress. Sendai, 1985, 38

Morphology: round cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:10,

optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 8

Plating efficiency: 10%

Applications: cell biology, somatic cell genetics, endocrinology

Collections: MWIGG