79f7Dv3g

Origin: Drosophila virilis (Insecta). 20h. Embryonic cells.

VII Europ. Drosophila Res. Conf. Finland, 1981, 23

Morphology: round cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:10,

optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 11 ( with one Y- chromosome)

Plating efficiency: 20%

Applications: cell biology, Drosophila virilis retrotransposone producent (Tv1)

Collections: MWIGG