75 e 7 vg 2

Origin: Drosophila melanogaster (Insecta), vestigial ( II- 67,0)

Drosophila Information Servise, 1977, 52:110

Morphology: round and spindle-shaped cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 5%

subculture procedure - cell detachment mechanically, split ratio 1:10,

optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 7

Plating efficiency: 5%

Other properties:

virus susceptibility: picornaviruses

isoenzymes phumarasa, GFGD, ADG.

genetical markers: ES s.

Applications: cell biology, virology, genetics of Somatic cells

Collections: MWIGG