67j 25D

Origin: Drosophila melanogaster (Insecta) 6-12 h embryonic cells.

Genetica (Russ) 1969,12:67; Intervertebr.Systems in vitro 1980. p 565.

Morphology: round and spindle-shaped cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 5- 10%

subculture procedure - cell detachment mechanically, split ratio 1:10, optimal population density 0.5x106 cells/ml

cryoconservation - growth medium, 10% DMSO 2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=8 ( female cells)

Plating efficiency: 10%

Other properties:

virus susceptibility: picornaviruses, DVX

isoenzymes PGD,G6PD, Fich, a -Gpdh

genetical markers: GPRT-, ES s, 8Agr, 6MPr.

Applications: cell biology, molecular and ecological genetics, endicrynology, cytogerontology,

reproduction of drosophila retrotransposones: MDG-1, MDG-3, copia, gypsy, 17,6 and 297.

Collections: MWIGG