67j25DT

Origin: Drosophila melanogaster (Insecta). Clonal line, embryonic cells.

Genetica (Russ) 1969, 12: 67-75; Ontogenez (Russ), 1971, 2: 259-303

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum - FBS 10%

subculture procedure -cell detachment mechanically, split ratio 1:10, optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cell/ ml in ampule.

Viability after cryoconservation: 90% (0 passage, due trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymologycal (GPGD) analysis

Karyology: tetraploid (4n=16 chromosomes)

Plating efficiency: 50%

Other properties:

virus susceptibility: picornaviruses

isoenzymes GPGD, G6PD

genetical markers: GPRT -; Es s.

Applications: cell biology, virology, biotechnology.

Collections: MWIGG