Origin:Drosophila melanogaster (Insecta), clonal line, embryonic cells.
Ontogenez (Russ.), 1971, 2: 304- 310.
Morphology: round cells
Mode of cultivation: monolayer
Conditions for cultivation: medium - C-46
serum FBS 10%
subculture procedure - cell detachment mechanically, split ratio 1:10, optimal population density 1.0x106 cells/ml
cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: triploid 3n = 12
Plating efficiency: 10%
virus susceptibility: picornaviruses, DVX
isoenzymes PGD, G6PD
genetical markers: GPRT-, 8AG r, 6MP r, ES s.
Applications: cell biology