67j25DK

Origin: Drosophila melanogaster (Insecta), clonal line, embryonic cells.

Ontogenez (Russ.), 1971, 2: 304- 310.

Morphology: round cells

Mode of cultivation: monolayer

Conditions for cultivation: medium - C-46

serum FBS 10%

subculture procedure - cell detachment mechanically, split ratio 1:10, optimal population density 1.0x106 cells/ml

cryoconservation - growth medium, 10% DMSO, 2.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: triploid 3n = 12

Plating efficiency: 10%

Other properties:

virus susceptibility: picornaviruses, DVX

isoenzymes PGD, G6PD

genetical markers: GPRT-, 8AG r, 6MP r, ES s.

Applications: cell biology

Collections: MWIGG