W. som.

Origin: Withania somnifera Dun. (Solanaceae) juvenile plant hypocotyl transformation

with pRi Agrobacterium rhizogenes, strain R-1601

Morphology: highly branching roots

Conditions for cultivation:  medium -  B 5 O

22-25oC, 90 rpm, liquid culture

subculture procedure - transferring root tips each 3 weeks

explant ~ 250 mg per 50 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 70%

Sterility: fungi and bacteria free

Species: root specific vitanolide biosynthesis, opine test

Plating efficiency:  non-cloning

Other properties:

root specific sesquiterpen lactones (vitanolides)

Applications: biotechnology

Collections: HRC