Trif. rep.

Origin: Trifolium repens L. (cež. Fabaceae) juvenile plant hypocotyl transformation

 with pRi Agrobacterium rhizogenes, strain 15834

Morphology: highly branching roots

Conditions for cultivation:  medium -  MS (1/2 N)

22-25oC, 90 rpm, liquid culture

subculture procedure - transferring root tips each 3 weeks

explant ~ 250 mg per 30 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 80%

Sterility: fungi and bacteria free

Species: opine test

Plating efficiency:  non-cloning

Applications: biotechnology

Collections: HRC