Origin: Tagetes patula (Compositae) juvenile plant hypocotyl transformation

with pRi Agrobacterium rhizogenes, strain R-1601

Morphology: highly branching roots

Conditions for cultivation:  medium -  MSO (1/2 N), B 5 O

22-25oC , 90 rpm, liquid culture

subculture procedure - transferring root tips each 3 weeks

explant ~ 250 mg per 30 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 80%

Sterility: fungi and bacteria free

Species: theophene biosynthesis, opine test

Plating efficiency:  non-cloning

Other properties:

root specific theophenes biosynthesis

contamination sensitivity: sensitive

Applications: biotechnology, dual culture with endomycorrhizal fungi

Collections: HRC