R.t.

Origin: Rubia tinctorum L. (c. Rubiaceae) juvenile plant hypocotyl transformation

with pRi Agrobacterium rhizogenes, strain R-1601

Morphology: highly branching roots

Conditions for cultivation:  medium -  B 5 O, MSO (1/2 N)

22-25oC, 90 rpm, liquid culture

subculture procedure - transferring root tips each 3 weeks

explant ~ 250 mg per 50 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 90%

Sterility: fungi and bacteria free

Species: Root specific anthraquinone biosynthesis, opine test

Plating efficiency:  non-cloning

Other properties:

Root specific anthraquinones

Applications: biotechnology, enzimology of anthraquinone biosynthesis

Collections: HRC