R.s.

Origin: Rauvolfia serpentina (L.) Benth. (Apocinaceae) juvenile plant leaves,

transformation with pRi Agrobacterium rhizogenes, strain 4

Morphology: highly branching roots

Conditions for cultivation:  medium -  Gamborg (B 5 O)

temperature 22-25o, rotary shaking - 90 rpm, liquid culture

subculture procedure - transferring root tips each 5 weeks

explant ~ 250 mg per 60 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 90%

Sterility: fungi and bacteria free

Species: species specific alkaloids; opine test

Plating efficiency:  non-cloning

Other properties:

indole alkaloid biosynthesis

Applications: biotechnology, enzimology of indole alkaloid biosynthesis

Collections: HRC