R.gr.

Origin: Ruta graveolens L. (Rutaceae) juvenile plant leaves transformation

with pRi Agrobacterium rhizogenes, strain 15834

Morphology: highly branching roots

Conditions for cultivation:  medium -  B 5 O

22-25oC, 90 rpm, liquid culture

subculture procedure - transferring root tips each 4 weeks

explant ~ 250 mg per 50 ml of nutrient medium

conservation  - maintenance on the solid medium at room temperature

Viability after conservation: 90%

Sterility: fungi and bacteria free

Species: root specific coumarine and alkaloids biosynthesis

Plating efficiency:  non-cloning

Other properties:

root specific furocoumarines and alkaloids

Applications: biotechnology, enzimology of coumarine and acridone alkaloid biosynthesis

Collections: HRC