On.ar. A-4

Origin: Ononis arvensis L. (Fabaceae) juvenile plant hypocotyl transformation

with pRi Agrobacterium rhizogenes, strain A 4

Morphology: branching roots

Conditions for cultivation:  medium -  B 5 O

22-25oC, 90 rpm, liquid culture

subculture procedure - transferring root tips each 4 weeks

explant ~ 250 mg per 30 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 60%

Sterility: fungi and bacteria free

Species: phenolic compound biosynthesis, opine test

Plating efficiency:  non-cloning

Other properties:

root specific phenolic compounds

contamination sensitivity: sensitive

Applications: biotechnology

Collections: HRC