Origin: Rhaponticum carthamoides (Wild.) = Leuzea carthamoides Wild.) (Asteraceae);

juvenile plant hypocotyl transformation with pRi Agrobacterium rhizogenes, strain 4

Morphology: branching roots

Conditions for cultivation:  medium - Murashige and Skoog (1/2 N) plus 0.5 mg/l IAA

22-25oC, 90 rpm, liquid culture

subculture procedure - transferring a root segment each 8 weeks

explant ~ 100 mg per 30 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 50%

Sterility: fungi and bacteria free

Species: ecdysone synthesis, opine test

Plating efficiency:  non-cloning

Other properties:

root specific ecdisteroids

contamination sensitivity: sensitive to bacterial contamination

Applications: biotechnology

Collections: HRC