H.an.

Origin: Helanthus annuus L. (Compositae) juvenile plant leaves transformation

with pRi Agrobacterium rhizogenes, strain R-1601

Morphology: highly branching roots

Conditions for cultivation:  medium - MSO (1/2 N), B 5 O

22-25oC, 90 rpm, liquid culture

subculture procedure - transferring root tips each 3 weeks

explant ~ 250 mg per 60 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 80%

Sterility: fungi and bacteria free

Species: opine test

Plating efficiency:  non-cloning

Other properties:

root specific coumarines and phenolic compounds

Applications: biotechnology, biochemistry of signaling compounds

Collections: HRC