Origin: Daucus carota L. (Umbellifereae) transformation

with pRi Agrobacterium rhizogenes, strain ATCC 15834

Morphology: highly branching roots

Conditions for cultivation:  medium - B 5 O

22-25oC, 90 rpm, liquid culture

subculture procedure - transferring root tips each 3 weeks

explant ~ 250 mg per 30 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 90%

Sterility: fungi and bacteria free

Species: opine test

Plating efficiency:  non-cloning

Applications: biotechnology, dual culture with endomycorrhizal fungi

Collections: HRC