Origin: Cucumis sativus L. (Cucurbitaceae) seedling cotyledones transformation

with pRi Agrobacterium rhizogenes, strain 8196

Morphology: highly branching thickened roots

Conditions for cultivation:  medium - B 5 O

22-25oC, 90 rpm, liquid culture

subculture procedure - transferring root tips each 3 weeks

explant ~ 250 mg per 30 ml of nutrient medium

conservation - maintenance on the solid medium at room temperature

Viability after conservation: 90%

Sterility: fungi and bacteria free

Species: opine test

Plating efficiency:  non-cloning

Other properties:

root specific cucurbitacides

Applications: biotechnology

Collections: HRC