XPA

Origin: human, SV 40 virus-transformed fibroblasts from xeroderma pigmentosum patients.

Mol.Cell Biol. 1987. 7: 3353-3357.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum - FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:3

cryoconservation - growth medium, 5-8%DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 69-79 chromosomes without

modal number, number of polyploid cells 1.8%.

Applications: genetics, tumorigenicity, cell biology.

Collections: SPBIC

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