Origin: human, SV 40 virus-transformed fibroblasts from xeroderma pigmentosum patients.
Mol.Cell Biol. 1987. 7: 3353-3357.
Mode of cultivation: monolayer
Conditions for cultivation: medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:3
cryoconservation - growth medium, 5-8%DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 69-79 chromosomes without
modal number, number of polyploid cells 1.8%.
Applications: genetics, tumorigenicity, cell biology.