HuTu 80

Origin: human, duodenum, adenocarcinoma

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or EMEM

serum - FBS 10%

other components - NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:1 - 1:3),

split ratio 1:3 cryoconservation - growth medium, 5-10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, variability in the range between 42-48 chromosomes, modal number

 of chromosomes 46, pseudodiploid, number of polyploid cells 0.4%,

number of markers - 3 (differential dye) (ATCC) .

Tumorigenicity: tumorigenic in nude mice

Other properties:

isoenzymes PGM3,1-2; PGM1,1-2; ES D,1; Me-2,2; AK 1,1; GLO-1,2; G6PD,B

Applications: tumorigenicity, cell biology

Collections: ATCC HTB 40; SPBIC.

 

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