U-937

Origin: human, histiocytic lymphoma (pleural effusion)

Int.J.Cancer 1976. 17: 565-577; J.Exp.Med. 1976. 143: 1528-1533; J.Immunol.

1980. 125: 463-465; Nature 1979. 279: 328-331.

Morphology: histiomonocitoid

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 2.0-9.0x105 cells/ml

cryoconservation - growth medium, 8-9%DMSO, 5.0106 cells/ml in ampule

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 59-65 chromosomes, modal number

of chromosomes 63, number of markers - 23 (differential dye), number of polyploid cells 0.2%.

Other properties:

virus susceptibility: HIV-1, HIV-2, herpes type 6.

IL-1 production.

Fc and C3 receptors.

Phagocytose antibody-coated erythrocytes and latex beads.

Applications: differentiation, virology, cell biology, tumorigenicity.

Collections: ATCC CRL 1593; DSM ACC 5; ECACC 85011440; 87010802;

ICLC HTL 94002; SPBII; SPBIC.

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