U-2 OS

Origin: human, osteosarcoma.

Int.J.Cancer 1967. 2: 434-447.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - RPMI 1640 or McCoy's 5a.

serum - FBS 10-15%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:5 - 1:10

cryoconservation - growth medium, 5-10% DMSO,

1.0-2.0106 cells/ml in ampule

Viability after cryoconservation: 92% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 67-80 chromosomes,

modal number of chromosomes 76 and 78-79, number of markers - 22 (differential dye), (ATCC).

Other properties:

isoenzymes PGM1, 1; PGM3, 2; ES D, 1; AK 1, 1; GLO-1, 2; G6PD, B.

Applications: tumorigenicity, cell biology.

Collections: ATCC HTB 96; SPBIC.

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