Origin: human, glioblastoma.
J. Cell Physiol. 1979. 99: 43-54.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM
serum - FBS 10%
other components - NEAA 1%, sodium pyruvate 1mM.
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:3 -1:6
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 46, modal number of chromosomes 128-132, number
of markers - 14-16 (differential dye), number of polyploid cells 1.3% (ATCC).
Applications: studies on the mechanisms for cessation of proliferation,
cell synchronisation in G1 phase and ageing.
Collections: ATCC CRL 1690; SPBIC.