T 98G

Origin: human, glioblastoma.

J. Cell Physiol. 1979. 99: 43-54.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1%, sodium pyruvate 1mM.

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:3 -1:6

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, modal number of chromosomes 128-132, number

of markers - 14-16 (differential dye), number of polyploid cells 1.3% (ATCC).

Applications: studies on the mechanisms for cessation of proliferation,

cell synchronisation in G1 phase and ageing.

Collections: ATCC CRL 1690; SPBIC.