Origin: human, bladder carcinoma.
Int. J. Cancer 1970.5: 310; Int. J.Cancer 1971. 8: 503; Int. J. Cancer 1973.11: 765;
Tissue Antigens. 1978.11:279.
Mode of cultivation: monolayer
Conditions for cultivation: medium - BME
serum - FBS 10%
subculture procedure - cells detach from flask using EDTA 0.02%
with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0õ105 cells/cm2
cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation: 86% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 77-99 chromosomes, modal number
of chromosomes 93 without markers (routine, differential dye, C-banding), there are
microchromosomes, number of polyploid cells 2.0%.
Isoenzymes G6PD,B; Ìå-2,2-1; PGM 3,1; FUC,2-1; PGM 1,1; ESD,1; ADA,1.
HLA cell line phenotype A (1,3); B (8,18); C (w2, w6), Ek-2.
Applications: virology, tumorigenicity.
Collections: ATCC HTB 4; MWIIW.