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Origin: human, bladder carcinoma.

Int. J. Cancer 1970.5: 310; Int. J.Cancer 1971. 8: 503; Int. J. Cancer 1973.11: 765;

Tissue Antigens. 1978.11:279.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum - FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0105 cells/cm2

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation: 86% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 77-99 chromosomes, modal number

of chromosomes 93 without markers (routine, differential dye, C-banding), there are

microchromosomes, number of polyploid cells 2.0%.

Tumorigenicity: tumorigenic

Other properties:

Isoenzymes G6PD,B; -2,2-1; PGM 3,1; FUC,2-1; PGM 1,1; ESD,1; ADA,1.

HLA cell line phenotype A (1,3); B (8,18); C (w2, w6), Ek-2.

Applications: virology, tumorigenicity.

Collections: ATCC HTB 4; MWIIW.

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