Origin: human, acute lymphoblastic bone marrow leukemia.
Mode of cultivation: suspension
Conditions for cultivation: medium - RPMI 1640
serum - FBS 10%
subculture procedure - optimal population density 2.0-5.0x105 cells/ml
cryoconservation - RPMI 1640 40%, FBS 10%,
glycerol 10%, 5.0-10.0х106 cells/ml in ampule
Viability after cryoconservation: 91% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=46, variability in the range between 39-47 chromosomes, modal number
of chromosomes 46, without markers (routine and differential dye, C -banding), number of polyploid cells 2.0%.
virus susceptibility: alphaviruses; Semliki Forest virus; Venezuelan equine encephalomyelitis;
Isoenzymes G 6 PD,8
Applications: virology .