T-1387

Origin: human, acute lymphoblastic bone marrow leukemia.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 2.0-5.0x105 cells/ml

cryoconservation - RPMI 1640 40%, FBS 10%,

glycerol 10%, 5.0-10.0х106 cells/ml in ampule

Viability after cryoconservation: 91% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, variability in the range between 39-47 chromosomes, modal number

of chromosomes 46, without markers (routine and differential dye, C -banding), number of polyploid cells 2.0%.

Other properties

virus susceptibility: alphaviruses; Semliki Forest virus; Venezuelan equine encephalomyelitis;

Isoenzymes G 6 PD,8

Applications: virology .

Collections: MWIIW

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