RT-4v

Origin: human, bladder papillary carcinoma, subline of RT-4.

Brit.J.Cancer 1970. 24: 746; Int.J.Cancer 1972. 10: 77; Tissue Antigens 1978. 11:279.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - BME

serum - FBS 10%

subculture procedure - cells detach from flask using EDTA 0.02%

 with 0.1 mg/ml chimopsin, split ratio 1:3-1:5, optimal population density 1.0105 cells/cm2

cryoconservation - BME 70%, FBS 20%, glycerol 10%, 3.0-5.0106 cells/ml in ampule

Viability after cryoconservation: 94% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 82-94 chromosomes, modal number

of chromosomes 88, Y-chromosome (C- and G-banding) and microchromosomes (routine dye) are presented.

Tumorigenicity: tumorigenic

Other properties:

isoenzymes G6PD,B; ME-M,1; PGM3,2-1; FUC,1; PGM1,2-1; ESD,2-1; AK1,1.

HLA cell line phenotype F (1, 3); B (12); Ek-2; Ek-5.

Applications: virology, tumorigenicity.

Collections: MWIIW.

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