RH

Origin: human, kidney.

Submitted by Brazilian Institute of Biology,1960 .

Morphology: epithelial-like

Mode of cultivation: monolayer

onditions for cultivation: medium - 199

serum - BS 10%

subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:5-1:6,

optimal population density 1.0-1.2x105 cells/ml

cryoconservation - growth medium, 10% glycerol, 2.0 106 cells/ml in ampule

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n=46, variability in the range between 45-87 chromosomes,

modal number of chromosomes 60-65.

Plating efficiency: 65%

Other properties:

virus susceptibility: polioviruses; Coxsackie A and B; ECHO; human adenoviruses.

Applications: virology.

Collections: ESCC

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