Origin: human, kidney.
Submitted by Brazilian Institute of Biology,1960 .
Mode of cultivation: monolayer
onditions for cultivation: medium - 199
serum - BS 10%
subculture procedure - cells detach from flask using EDTA 0.02%, split ratio 1:5-1:6,
optimal population density 1.0-1.2x105 cells/ml
cryoconservation - growth medium, 10% glycerol, 2.0 õ106 cells/ml in ampule
Viability after cryoconservation: 80% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n=46, variability in the range between 45-87 chromosomes,
modal number of chromosomes 60-65.
Plating efficiency: 65%
virus susceptibility: polioviruses; Coxsackie A and B; ECHO; human adenoviruses.