Origin: human, embryonic rhabdomyosarcoma.
J. Virol. 1967. 1: 326; Cancer 1969. 24: 520-526.
Morphology: spindle-shaped cells and large multinucleated cells.
Mode of cultivation: monolayer
Conditions for cultivation: medium - DMEM or EMEM with twice the standard concentrations of amino acids and vitamins.
serum - FBS or BS 10%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:2), split ratio 1:2, optimal population density 4.0õ104 cells/cm2
cryoconservation - growth medium (may add 30% BS),
5-10% DMSO or glycerol, 1.5-4.0õ106 cells/ml in ampule
Viability after cryoconservation: 70-80% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological ( LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 45-49 chromosomes, modal number
of chromosomes 48, some cells have microchromosomes, number of polyploid cells 8.0%.
Plating efficiency: less 1% (ATCC), 50% (ESCC).
virus susceptibility: poliovirus 1, vesic. stomatitis, herpes simplex, vaccinia, cytomegalovirus,
Isoenzymes G6PD, B.
Myoglobin secretion; myoglobin and myosin-ATPase activity.
Applications: differentiation, biochemistry, genetics, tumorigenicity, cell biology.
Collections: ATCC CCL 136; ECACC 85111502; MWIIW; SPBII; ESCC; SPBIC.