RD

Origin: human, embryonic rhabdomyosarcoma.

J. Virol. 1967. 1: 326; Cancer 1969. 24: 520-526.

Morphology: spindle-shaped cells and large multinucleated cells.

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM or EMEM with twice the standard concentrations of amino acids and vitamins.

serum - FBS or BS 10%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:2), split ratio 1:2, optimal population density 4.0104 cells/cm2

cryoconservation - growth medium (may add 30% BS),

5-10% DMSO or glycerol, 1.5-4.0106 cells/ml in ampule

Viability after cryoconservation: 70-80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological ( LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 45-49 chromosomes, modal number

of chromosomes 48, some cells have microchromosomes, number of polyploid cells 8.0%.

Plating efficiency: less 1% (ATCC), 50% (ESCC).

Other properties:

virus susceptibility: poliovirus 1, vesic. stomatitis, herpes simplex, vaccinia, cytomegalovirus,

parainfluenza, rotaviruses.

Isoenzymes G6PD, B.

Myoglobin secretion; myoglobin and myosin-ATPase activity.

Applications: differentiation, biochemistry, genetics, tumorigenicity, cell biology.

Collections: ATCC CCL 136; ECACC 85111502; MWIIW; SPBII; ESCC; SPBIC.

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