Raji

Origin: human, Burkitt lymphoma

Lancet 1964. 1: 238; J.Bact. 1965. 89: 252; J. Clin. Pathol.1965. 18: 261;

J.Natl.Cancer Inst. 1965. 34: 231; J.Natl.Cancer Inst. 1966. 37: 547; Trans. NY Acad. Sci. 1966. 29: 61.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 3.0-9.0105 cells/ml

cryoconservation - growth medium (may add 40% FBS), 10% DMSO,

5.0-10.0106 cells/ml in ampule

Viability after cryoconservation: 78-88% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis (LDH, G6PD)

Karyology: 2n= 46, variability in the range between 43-48 chromosomes, modal number

of chromosomes 48, number of markers - 8, number of polyploid cells 6.0%

Plating efficiency: 40% (MWIIW)

Other properties:

virus susceptibility: simian retrovirus D, arboviruses.

Isoenzymes G6PD, B.

HLA cell line phenotype (1, 3).

Erythrocyte rosette tests: E, 0; EA, 1%; EAC, 34%.

Positive for EBNA, but does not contain the EBV.

Applications: B-cell differentiation, immunology, antitumor testing, virology.

Collections: ATCC CCL 86; ECACC 85011429; MWIIW; SPBIC.

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