Origin:human, pancreatic carcinoma
Int.J.Cancer 1975. 15: 741-747.
Mode of cultivation: monolayer
Conditions for cultivation: medium - DMEM
serum - FBS 10%
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:1),
split ratio 1:2 - 1:4, optimal population density 2.0-4.0õ104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 4.0-5.0õ106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 46, modal number of chromosomes 61 and 63, number
of markers - 4 (differential dye), number of polyploid cells 8.5% (ATCC)
isoenzymes G6PD, B.
Collections: ATCC CRL 1469: ECACC 87092802; SPBIC.