PANC-1

Origin: human, pancreatic carcinoma

Int.J.Cancer 1975. 15: 741-747.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - DMEM

serum - FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:1),

split ratio 1:2 - 1:4, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 4.0-5.0106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, modal number of chromosomes 61 and 63, number

of markers - 4 (differential dye), number of polyploid cells 8.5% (ATCC)

Other properties:

isoenzymes G6PD, B.

Applications: tumorigenicity:

Collections: ATCC CRL 1469: ECACC 87092802; SPBIC.

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