PA-1

Origin: human, ovarian teratocarcinoma, ascitic fluid

J.Natl.Cancer Inst. 1974. 52: 921; In Vitro 1974. 10: 382; Int.J.Cancer 1980. 25: 19-32.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM or DMEM

serum - FBS 10%

other components - NEAA 1% (EMEM)

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),

split ratio 1:3 - 10, optimal population density 1.0-3.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0-2.0106 cells/ml in ampule

Viability after cryoconservation: 87% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 33-46 chromosomes, modal number

of chromosomes 46, pseudodiploid, number of markers - 1 (differential dye, ATCC), number of polyploid cells 0.4%.

Tumorigenicity: tumorigenic in nude mice

Other properties:

chemotaxis, chemoinvasion, matrigel invasion.

Applications: tumorigenicity, cell biology.

Collections: ATCC CRL 1572; ECACC 90013101; ICLC HTL 97002; SPBIC.

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