Origin: human, ovarian teratocarcinoma, ascitic fluid
J.Natl.Cancer Inst. 1974. 52: 921; In Vitro 1974. 10: 382; Int.J.Cancer 1980. 25: 19-32.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM or DMEM
serum - FBS 10%
other components - NEAA 1% (EMEM)
subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),
split ratio 1:3 - 10, optimal population density 1.0-3.0õ104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 1.0-2.0õ106 cells/ml in ampule
Viability after cryoconservation: 87% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 33-46 chromosomes, modal number
of chromosomes 46, pseudodiploid, number of markers - 1 (differential dye, ATCC), number of polyploid cells 0.4%.
Tumorigenicity: tumorigenic in nude mice
chemotaxis, chemoinvasion, matrigel invasion.
Applications: tumorigenicity, cell biology.
Collections: ATCC CRL 1572; ECACC 90013101; ICLC HTL 97002; SPBIC.