P3H3

Origin: human, Burkitt lymphoma

J. Clin. Path. 1965. 18: 261; J. Virol. 1967.1: 1045; Proc.Soc.Exp.Biol.Med. 1967.124: 107.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 2.0-5.0x105 cells/ml

cryoconservation - RPMI 1640 40%, FBS 50%, glycerol 10%, 5.0-10.0106 cells/ml in ampule

Viability after cryoconservation: 77% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological ( LDH, G6PD) analysis

Karyology: 2n=46, variability in the range between 41-51 chromosomes, modal number

of chromosomes 48, number of polyploid cells 14.0%

Other properties:

isoenzymes G6PD, B.

Contain and produce EBV

Applications: virology , the cells used for the indication of IgG and IgA,

specific against early antigen (EA) of EBV

Collections: MWIIW

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