Origin: human, Burkitt lymphoma
J. Clin. Path. 1965. 18: 261; J. Virol. 1967.1: 1045; Proc.Soc.Exp.Biol.Med. 1967.124: 107.
Mode of cultivation: suspension
Conditions for cultivation: medium - RPMI 1640
serum - FBS 10%
subculture procedure - optimal population density 2.0-5.0x105 cells/ml
cryoconservation - RPMI 1640 40%, FBS 50%, glycerol 10%, 5.0-10.0õ106 cells/ml in ampule
Viability after cryoconservation: 77% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological ( LDH, G6PD) analysis
Karyology: 2n=46, variability in the range between 41-51 chromosomes, modal number
of chromosomes 48, number of polyploid cells 14.0%
isoenzymes G6PD, B.
Contain and produce EBV
Applications: virology , the cells used for the indication of IgG and IgA,
specific against early antigen (EA) of EBV