Origin: human, renal carcinoma

Folia Biol. 1988. 34: 308.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM (Hanks' BSS)

serum - FBS 10%

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3), split ratio 1:2

cryoconservation - growth medium, 8-10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, modal number of chromosomes 75, number of markers - 2 (differential dye)

Tumorigenicity: non tumorigenic in nude mice

Applications: tumorigenicity, cell biology

Collections: SPBIC