Origin: human, lymphoblasts, peripheral blood from an apparently healthy donor.
Int.J.Cancer 1970. 6: 436-449.
Mode of cultivation: suspension
Conditions for cultivation: medium - RPMI 1640
serum - FBS 10%
subculture procedure - optimal population density 4.0-5.0๕105 cells/ml
cryoconservation - growth medium, 10% DMSO, 3.0-5.0x106 cells/ml in ampule
Viability after cryoconservation: 82% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 40-51 chromosomes,
modal number of chromosomes 48 (ATCC)
Plating efficiency: the cells cannot be plated (ATCC)
isoenzymes G6PD, B.
Erythrocyte rosette tests: E, 0; EA, 2%; EAC, 60%.
Each cell contains an average of 60 EBN genome copies.
Applications: virology, cell biology, biochemistry, immunology.
Collections: ภาัั CCL 214: ECACC 89111414; SPBII; SPBIC.