Origin: human, mixed heart's and peripheral blood's lymphocytes of patient with T-cell leukemia.
Submitted from All-Union Research Institute of Pure Biopreparations, Leningrad, 1990.
Mode of cultivation: suspension
Conditions for cultivation: medium - RPMI 1640
serum - FBS 10%
subculture procedure - optimal population density 5.0-9.0õ105 cells/ml
cryoconservation - growth medium, 10% DMSO, 3.0-5.0x106 cells/ml in ampule
Viability after cryoconservation: 50% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 46, variability in the range between 80-87 chromosomes, modal number o
f chromosomes 83, number of polyploid cells 0.4%.
virus susceptibility: HIV.