MT-4

Origin: human, mixed heart's and peripheral blood's lymphocytes of patient with T-cell leukemia.

Submitted from All-Union Research Institute of Pure Biopreparations, Leningrad, 1990.

Morphology: lymphoid

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 5.0-9.0105 cells/ml

cryoconservation - growth medium, 10% DMSO, 3.0-5.0x106 cells/ml in ampule

Viability after cryoconservation: 50% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, variability in the range between 80-87 chromosomes, modal number o

f chromosomes 83, number of polyploid cells 0.4%.

Other properties:

virus susceptibility: HIV.

Applications: virology

Collections: SPBIC.

Up