Origin: human, embryonic lung.
Nature 1970. 227: 168-170.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM
serum - FBS 10%
other components - NEAA 1%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:2 - 1:6, optimal population density 2.0-4.0õ104 cells/cm2
cryoconservation - growth medium, FBS 5%, 8%DMSO, 3.0-4.0õ106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymological (LDH, G6PD) analysis
Karyology: 2n= 46, variability in the range between 44-48 chromosomes, modal number
of chromosomes 46, normal human karyotype, (46, XY) number of polyploid cells 3.5%.
Plating efficiency: less 1% (ATCC)
Tumorigenicity: non tumorigenic
virus susceptibility: herpes simplex, poliovirus 1, vesic. stomatitis (Indiana).
Isoenzymes G6PD, B.
Finite lifetime culture, cells are capable of attaining 42-48 population doubling before onset of the decline in proliferation.
Applications: cell biology, virology
Collections: ATCC CCL 171; ECACC 84101801; ICLC HL 95001; ESCC; SPBIC.