MRC-5

Origin: human, embryonic lung.

Nature 1970. 227: 168-170.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1%

subculture procedure - cells detach from flask using trypsin 0.25%:

EDTA 0.02% (1:3), split ratio 1:2 - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, FBS 5%, 8%DMSO, 3.0-4.0106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymological (LDH, G6PD) analysis

Karyology: 2n= 46, variability in the range between 44-48 chromosomes, modal number

of chromosomes 46, normal human karyotype, (46, XY) number of polyploid cells 3.5%.

Plating efficiency: less 1% (ATCC)

Tumorigenicity: non tumorigenic

Other properties:

virus susceptibility: herpes simplex, poliovirus 1, vesic. stomatitis (Indiana).

Isoenzymes G6PD, B.

Finite lifetime culture, cells are capable of attaining 42-48 population doubling before onset of the decline in proliferation.

Applications: cell biology, virology

Collections: ATCC CCL 171; ECACC 84101801; ICLC HL 95001; ESCC; SPBIC.

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