MOLT-4

Origin: human, T-lymphoblastic leukemia, peripheral blood.

J.Natl.Cancer Inst. 1972. 49: 891-895; J.Immunol. 1982. 129: 2504-2510; Int.J.Immunopharmacol.

1988. 10: 907-911; Glukhova L.A. PhD Thesis; SPBIC, St.Petersburg. 1992.

Morphology: lymphoblast-like

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 2.0-5.0105 cells/ml

cryoconservation - RPMI 1640 40%, FBS 50%, glycerol 10%, 5.0-10.0106 cells/ml in ampule

Viability after cryoconservation: 94% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological and isoenzymologycal (LDH, G6PD) analysis

Karyology 2n= 46, variability in the range between 77-100 chromosomes, modal number

of chromosomes 97, number of markers - 6 (differential dye), number of polyploid cells 2.0%.

Tumorigenicity: tumorigenic in nude mice

Other properties:

virus susceptibility: measles, a -viruses

Terminal deoxynucleotidyl transferase activity is high.

The cells form rosettes with sheep erythrocytes.

Applications: biochemistry, cytotoxicity, differentiation, virology, tumorigenicity, immunology

Collections: ATCC CRL 1582; ECACC 85011413; MWIIW; SPBIC.

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