Origin: human, T-lymphoblastic leukemia, peripheral blood.
J.Natl.Cancer Inst. 1972. 49: 891-895; J.Immunol. 1982. 129: 2504-2510; Int.J.Immunopharmacol.
1988. 10: 907-911; Glukhova L.A. PhD Thesis; SPBIC, St.Petersburg. 1992.
Mode of cultivation: suspension
Conditions for cultivation: medium - RPMI 1640
serum - FBS 10%
subculture procedure - optimal population density 2.0-5.0õ105 cells/ml
cryoconservation - RPMI 1640 40%, FBS 50%, glycerol 10%, 5.0-10.0õ106 cells/ml in ampule
Viability after cryoconservation: 94% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological and isoenzymologycal (LDH, G6PD) analysis
Karyology 2n= 46, variability in the range between 77-100 chromosomes, modal number
of chromosomes 97, number of markers - 6 (differential dye), number of polyploid cells 2.0%.
Tumorigenicity: tumorigenic in nude mice
virus susceptibility: measles,a -viruses
Terminal deoxynucleotidyl transferase activity is high.
The cells form rosettes with sheep erythrocytes.
Applications: biochemistry, cytotoxicity, differentiation, virology, tumorigenicity, immunology
Collections: ATCC CRL 1582; ECACC 85011413; MWIIW; SPBIC.