MOLT-3

Origin: human, T-lymphoblastic leukemia, peripheral blood.

J.Natl.Cancer Inst. 1972. 49: 891-895.

Morphology: lymphoid

Mode of cultivation: suspension

Conditions for cultivation: medium - RPMI 1640

serum - FBS 10%

subculture procedure - optimal population density 5.0-6.0105 cells/ml

cryoconservation - growth medium, 10% DMSO, 5.0106 cells/ml in ampule

Viability after cryoconservation: 80% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46 - modal number of chromosomes 98, number of markers - 4 (differential dye),

number of polyploid cells 2%.

Other properties:

virus susceptibility: HIV.

The cells form rosettes with sheep erythrocytes.

Applications: tumorigenicity, virology

Collections: ATCC CRL 1552; DSM ACC 84; ECACC 90021901; SPBIC.

Up