Origin: human, T-lymphoblastic leukemia, peripheral blood.
J.Natl.Cancer Inst. 1972. 49: 891-895.
Mode of cultivation: suspension
Conditions for cultivation: medium - RPMI 1640
serum - FBS 10%
subculture procedure - optimal population density 5.0-6.0õ105 cells/ml
cryoconservation - growth medium, 10% DMSO, 5.0õ106 cells/ml in ampule
Viability after cryoconservation: 80% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 46 - modal number of chromosomes 98, number of markers - 4 (differential dye),
number of polyploid cells 2%.
virus susceptibility: HIV.
The cells form rosettes with sheep erythrocytes.
Applications: tumorigenicity, virology
Collections: ATCC CRL 1552; DSM ACC 84; ECACC 90021901; SPBIC.