MG-63

Origin: human, osteosarcoma

Antimicrob. Agents Chemother. 1977. 12: 11-15.

Morphology: fibroblast-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1%

subculture procedure - cells detach from flask using trypsin 0.25%:

 EDTA 0.02% (1:3), split ratio 1:3 - 1:6,

 optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 90% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n= 46, variability in the range between 59-65 chromosomes, modal number

of chromosomes 63, number of markers - 18-19 (differential dye, ATCC), number of polyploid cells - 2%.

Applications: biotechnology (interferon production), cell biology

Collections: CRL 1427, ECACC 86051601; SPBIC.

Up