Origin: human, osteosarcoma
Antimicrob. Agents Chemother. 1977. 12: 11-15.
Mode of cultivation: monolayer
Conditions for cultivation: medium - EMEM
serum - FBS 10%
other components - NEAA 1%
subculture procedure - cells detach from flask using trypsin 0.25%:
EDTA 0.02% (1:3), split ratio 1:3 - 1:6,
optimal population density 2.0-4.0õ104 cells/cm2
cryoconservation - growth medium, 10% DMSO, 1.0x106 cells/ml in ampule
Viability after cryoconservation: 90% (0 passage, dye trypan blue)
Sterility: tests for bacteria, fungi and mycoplasma were negative
Species: karyological analysis
Karyology: 2n= 46, variability in the range between 59-65 chromosomes, modal number
of chromosomes 63, number of markers - 18-19 (differential dye, ATCC), number of polyploid cells - 2%.
Applications: biotechnology (interferon production), cell biology
Collections: ÀÒÑÑ CRL 1427, ECACC 86051601; SPBIC.