Origin: human, breast adenocarcinoma (pleural effusion)

J.Natl.Cancer Inst. 1973. 51: 1409-1416.

Morphology: epithelial-like

Mode of cultivation: monolayer

Conditions for cultivation: medium - EMEM

serum - FBS 10%

other components - NEAA 1%, sodium pyruvate 1mM, bovine insulin 10 m /ml.

subculture procedure - cells detach from flask using trypsin 0.25%: EDTA 0.02% (1:3),

split ratio 1:2 - 1:6, optimal population density 2.0-4.0104 cells/cm2

cryoconservation - growth medium, 8-9%DMSO, 1.0x106 cells/ml in ampule

Viability after cryoconservation: 94% (0 passage, dye trypan blue)

Sterility: tests for bacteria, fungi and mycoplasma were negative

Species: karyological analysis

Karyology: 2n=46, variability in the range between 67-87 chromosomes, modal number

of chromosomes 79-82, number of markers 2, large acrocentric and submetacentric

chromosomes (routine dye), 29-34 (differential dye, ATCC), number of polyploid cells 0.6%.

Tumorigenicity: tumorigenic in nude mice

Other properties:

isoenzymes PGM3, 1-2; PGM1, 2; ES D, 1; AK 1, 1; GLO-1, 1-2; G6PD, B. Estrogen receptor positive.

Estradiol synthesis.

Cells may carry B- or C-type virus.

The capability of forming domes.

Applications: receptor study, chemotherapeutic agents, tumorigenicity, cell biology, virology.

Collections: ATCC HTB 22; ECACC 86012803; ICLC HTL 95021; SPBIC